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| Registros recuperados: 35 | |
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| Han Ching, Lucay; Hadjadj, Annie; Sinquin, Corinne. |
| Les mollusques congelés représentent une part de plus en plus importante de nos importations de produits de la mer. Destinées à la consommation directe ou à la transformation, les noix de coquilles Saint Jacques congelées sont importées du Japon, du Royaume Uni ou d'Australie, en général glazurées individuellement, et souvent après trempage préalable destiné à les faire absorber une certaine quantité d'eau. C'est ainsi que l'eau totale ajoutée à la noix (glaçage + trempage) peut représenter 50 % du poids du produit avant traitement. A la demande conjointe de l'administration et de la profession, une méthode de détermination de l'eau de glaçage ajoutée a été mise au point au sein d'un groupe de travail animé par la FICUR. Dans ce même contexte, le besoin... |
| Tipo: Text |
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| Ano: 1987 |
URL: http://archimer.ifremer.fr/doc/00099/20988/18614.pdf |
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| Colliec-jouault, Sylvia; Chevolot, Lionel; Helley, Dominique; Ratiskol, Jacqueline; Bros, Andrée; Sinquin, Corinne; Roger, Olivier; Fischer, Anne-marie. |
| A new low-molecular-weight 'heparin-like' component was obtained from an exopolysaccharide produced by a mesophilic strain found in deep-sea hydrothermal vents. Data concerning the structure of the native high-molecular-weight exopolysaccharide (10(6) g/mol, 10% sulfate content) are reported for the first time. Two depolymerization processes were used to obtain low-molecular-weight (24-35 x 10(3) g/mol) oversulfated fractions (sulfate content 20 or 40%), Nuclear magnetic resonance studies indicated that after sulfation (40%), the low-molecular-weight fraction obtained by free radical depolymerization was less sulfated in the 6-O-position than the fraction depolymerized by acid hydrolysis. The free radical depolymerized product also had sulfated residues in... |
| Tipo: Text |
Palavras-chave: Sulfation; Sulfated polysaccharide; Serpin; Heparin like; Depolymerization; Anticoagulant activity; Affinity co electrophoresis. |
| Ano: 2001 |
URL: http://archimer.ifremer.fr/doc/2001/publication-2111.pdf |
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| Roger, Olivier; Colliec-jouault, Sylvia; Ratiskol, Jacqueline; Sinquin, Corinne; Guezennec, Jean; Fischer, A; Chevolot, Lionel. |
| Three sulphated polysaccharides labelled at the reducing end with three different chemical treatments (2-aminopyridine (2-AP), 8-amino-1,3,6-trisulphonic acid (ANTS) and 6-(biotinyl)-aminocaproyl-hydrazide (BACH)) were compared. Molecular weight, chemical composition, nuclear magnetic resonance features and anticoagulant activity expressed as activated partial thromboplastin time were studied to determine whether structural and biological properties were retained or not after labelling. Partial depolymerisation and reduced biological activity were observed when reductive amination was performed with 2-AP. The initial properties were better retained with ANTS, and BACH did not modify the anticoagulant and structural properties. (C) 2002 Elsevier Science... |
| Tipo: Text |
Palavras-chave: Anticoagulant activity; Reductive amination; Fluorescent tagging; Fucan; GAG; Sulphated polysaccharide. |
| Ano: 2002 |
URL: http://archimer.ifremer.fr/doc/2002/publication-2113.pdf |
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| Colliec-jouault, Sylvia; Zanchetta, P; Helley, D; Ratiskol, Jacqueline; Sinquin, Corinne; Fischer, A. M.; Guezennec, Jean. |
| Bacterial polysaccharides offer fascinating potential applications for the pharmaceutical industry. Although many known marine bacteria produce exopolysaccharides (EPS), continuation in looking for new polysaccharide-producing micro-organisms is promising. Marine bacteria, isolated from deep-sea hydrothermal vents, have demonstrated their ability to produce in aerobic conditions, unusual EPS. With the aim of discovering biological activities, EPS presenting different structural features were studied. An EPS secreted by Vibrio, diabolicus was evaluated on the restoration of bone integrity in experimental model and was demonstrated to be a strong bone-healing material. Another EPS produced by Alteromonas infernus was modified in order to obtain new... |
| Tipo: Text |
Palavras-chave: Hydrothermal vents; Exopolysaccharides; Bone healing; Biomimetic; Anticoagulant activity; Sources hydrothermales; Exopolysaccharide; Cicatrisation osseuse; Biomimétique; Activité anticoagulante. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/publication-2117.pdf |
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| Matou, Sabine; Colliec-jouault, Sylvia; Galy Fauroux, Isabelle; Ratiskol, Jacqueline; Sinquin, Corinne; Guezennec, Jean; Fischer, Anne-marie; Helley, Dominique. |
| The aim of this study was to determine the angiogenic properties of an oversulfated exopolysaccharide (OS-EPS) derived from a polysaccharide secreted by the mesophilic bacterium Alteromonas infernus. We compared the effect of this OS-EPS with that of a non-oversulfated exopolysaccharide (EPS) on human umbilical vein endothelial cell (HUVEC) proliferation, migration and differentiation induced by basic fibroblast growth factor (FGF-2) or vascular endothelial growth factor (VEGF). OS-EPS enhanced HUVEC proliferation by 58% when used alone, and by respectively 30% and 70% in the presence of FGF-2 and VEGF. OS-EPS also increased the density of tubular structures on Matrigel in the presence of FGF-2 or VEGF. Vascular tube fort-nation was related to alpha(6)... |
| Tipo: Text |
Palavras-chave: Matrigel; Collagen gel; Alpha 6 integrin subunit; Angiogenic growth factor; Polysaccharide; Angiogenesis. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-2118.pdf |
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| Petit, Anne-celine; Noiret, N; Sinquin, Corinne; Ratiskol, Jacqueline; Guezennec, Jean; Colliec-jouault, Sylvia. |
| Free-radical depolymerization with metallic catalysts has been investigated to depolymerize an exopolysaccharide (EPS), with a high molecular weight (> 10(6) g/Mol), produced by the bacterium Alteromonas macleodii subsp. fijiensis biovar deepsane. Three metals (copper, zinc and manganese) were used either alone or mixed. These metals led to the depolymerization of the high molecular weight bacterial polysaccharide with different efficiency. This study specifies some conditions required to produce, with good reproducibility and yield, EPS derivatives of reduced molecular weight ranging from 100,000 to 20,000 g/mol. The more influential parameters are specified. This is a first study to find suitable industrial conditions in the presence of these metallic... |
| Tipo: Text |
Palavras-chave: Bacterial exopolysaccharide; Free radical depolymerization; H202; Metal. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-2146.pdf |
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| Rigouin, Coraline; Delbarre Ladrat, Christine; Sinquin, Corinne; Colliec-jouault, Sylvia; Dion, M. |
| To find biocatalyst allowing depolymerization of new polysaccharides, one needs to get a sensitive and well adapted method to a screening program. This led us to compare biochemical methods of detection of the depolymerization. Currently used methods such as reducing sugars assays, double bond monitoring or molecular weight determination were tested to follow the kinetic of depolymerization with different enzyme / polysaccharide couples. The range of concentrations of assorted enzymes allowed us to identify the most sensitive and appropriate method to detect polysaccharide degradation. Reducing sugars assays are quantitative, sensitive and almost usable with all kind of polysaccharide but some compounds may interfere with them. When polysaccharide is... |
| Tipo: Text |
Palavras-chave: Detection; Enzyme; Depolymerization; Carbohydrate. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4723.pdf |
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| Velasco, C. Ruiz; Baud'Huin, M.; Sinquin, Corinne; Maillasson, M.; Heymann, D.; Colliec-jouault, Sylvia; Padrines, M.. |
| The growth and differentiation of bone cells is controlled by various factors which can be modulated by heparan sulphates. Here, we investigated the effects of an oversulphated exopolysaccharide (OS-EPS) on bone. We compared the effect of this compound with that of a native exopolysaccharide (EPS). Long-term administration of OS-EPS causes cancellous bone loss in mice due, in part, to an increase in the number of osteoclasts lining the trabecular bone surface. No significant difference in cancellous bone volume was found between EPS-treated mice and age-matched control mice, underlying the importance of sulphation in trabecular bone loss. However, the mechanism sustaining this osteoporosis was unclear. To clarify OS-EPS activities, we investigated the... |
| Tipo: Text |
Palavras-chave: Bone metabolism; Bone remodeling; Exopolysaccharide; Glycosaminoglycan; Heparin. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00032/14300/15749.pdf |
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| Bouhlal, Rhimou; Haslin, Camille; Chermann, Jean-claude; Colliec-jouault, Sylvia; Sinquin, Corinne; Simon, Gaelle; Cerantola, Stephane; Riadi, Hassane; Bourgougnon, Nathalie. |
| Water-soluble sulfated polysaccharides isolated from two red algae Sphaerococcus coronopifolius (Gigartinales, Sphaerococcaceae) and Boergeseniella thuyoides (Ceramiales, Rhodomelaceae) collected on the coast of Morocco inhibited in vitro replication of the Human Immunodeficiency Virus (HIV) at 12.5 mu g/mL. In addition, polysaccharides were capable of inhibiting the in vitro replication of Herpes simplex virus type 1 (HSV-1) on Vero cells values of EC(50) of 4.1 and 17.2 mu g/mL, respectively. The adsorption step of HSV-1 to the host cell seems to be the specific target for polysaccharide action. While for HIV-1, these results suggest a direct inhibitory effect on HIV-1 replication by controlling the appearance of the new generations of virus and... |
| Tipo: Text |
Palavras-chave: Herpes simplex virus; Human immunodeficiency virus; Antiviral activity; Sulfated polysaccharide; S. coronopifolius; B. thuyoides; Chemical composition. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00060/17120/14631.pdf |
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| Rederstorff, Emilie; Fatimi, Ahmed; Sinquin, Corinne; Ratiskol, Jacqueline; Merceron, Christophe; Vinatier, Claire; Weiss, Pierre; Colliec-jouault, Sylvia. |
| Polysaccharides are highly heat-sensitive macromolecules, so high temperature treatments are greatly destructive and cause considerable damage, such as a great decrease in both viscosity and molecular weight of the polymer. The technical feasibility of the production of exopolysaccharides by deep-sea bacteria Vibrio diabolicus and Alteromonas infernus was previously demonstrated using a bioproduct manufacturing process. The objective of this study was to determine which sterilization method, other than heat sterilization, was the most appropriate for these marine exopolysaccharides and was in accordance with bioprocess engineering requirements. Chemical sterilization using low-temperature ethylene oxide and a mixture of ionized gases (plasmas) was compared... |
| Tipo: Text |
Palavras-chave: Marine biotechnology; Polysaccharides; Sterilization procedures; Characterization; Molecular weight distribution; Rheology. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00027/13776/10913.pdf |
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| Rederstorff, Emilie; Weiss, Pierre; Sourice, S.; Pilet, P.; Xie, F.; Sinquin, Corinne; Colliec-jouault, Sylvia; Guicheux, Jerome; Laib, S.. |
| Natural polysaccharides are attractive compounds with which to build scaffolds for bone and cartilage tissue engineering. Here we tested two non-standard ones, HE800 and GY785, for the two-dimensional (2-D) and three-dimensional (3-D) culture of osteoblasts (MC3T3-E1) and chondrocytes (C28/I2). These two glycosaminoglycan-like marine exopolysaccharides were incorporated into an injectable silylated hydroxypropylmethylcellulose-based hydrogel (Si-HPMC) that has already shown its suitability for bone and cartilage tissue engineering. Results showed that, similarly to hyaluronic acid (HA) (the control), HE800 and GY785 significantly improved the mechanical properties of the Si-HPMC hydrogel and induced the attachment of MC3T3-E1 and C28/I2 cells when these... |
| Tipo: Text |
Palavras-chave: Glycosaminoglycan; Hydrogel; Polysaccharide; Bone and cartilage tissue engineering; In vitro test. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00030/14090/15747.pdf |
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| Senni, Karim; Pereira, Jessica; Gueniche, Farida; Delbarre-ladrat, Christine; Sinquin, Corinne; Ratiskol, Jacqueline; Godeau, Gaston; Fischer, Anne-marie; Helley, Dominique; Colliec-jouault, Sylvia. |
| The therapeutic potential of natural bioactive compounds such as polysaccharides, especially glycosaminoglycans, is now well documented, and this activity combined with natural biodiversity will allow the development of a new generation of therapeutics. Advances in our understanding of the biosynthesis, structure and function of complex glycans from mammalian origin have shown the crucial role of this class of molecules to modulate disease processes and the importance of a deeper knowledge of structure-activity relationships. Marine environment offers a tremendous biodiversity and original polysaccharides have been discovered presenting a great chemical diversity that is largely species specific. The study of the biological properties of the... |
| Tipo: Text |
Palavras-chave: Marine bacteria; Marine algae; Exopolysaccharides; Sulfated polysaccharides; Structure; Chemical modification; Biological activity; Blue biotechnology; Cell therapy; Tissue engineering. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00048/15894/13316.pdf |
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| Merceron, Christophe; Portron, Sophie; Vignes-colombeix, Caroline; Rederstorff, Emilie; Masson, Martial; Lesoeur, Julie; Sourice, Sophie; Sinquin, Corinne; Colliec-jouault, Sylvia; Weiss, Pierre; Vinatier, Claire; Guicheux, Jerome. |
| Mesenchymal stem cells (MSCs) are considered as an attractive source of cells for cartilage engineering due to their availability and capacity for expansion and multipotency. Differentiation of MSC into chondrocytes is crucial to successful cartilage regeneration and can be induced by various biological agents, including polysaccharides that participate in many biological processes through interactions with growth factors. Here, we hypothesize that growth factor-induced differentiation of MSC can be increased by chemically oversulfated marine polysaccharides. To test our hypothesis, human adipose tissue-derived MSCs (hATSCs) were cultured in pellets with transforming growth factor (TGF)-beta 1-supplemented chondrogenic medium containing either the... |
| Tipo: Text |
Palavras-chave: Cartilage; Adipose-derived mesenchymal stem cells; Polysaccharides; Transforming growth factor-beta. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00071/18227/17457.pdf |
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| Le Costaouec, Tinaig; Cerantola, Stephane; Ropartz, D.; Ratiskol, Jacqueline; Sinquin, Corinne; Colliec-jouault, Sylvia; Boisset, Claire. |
| Some marine bacteria collected around deep-sea hydrothermal vents are able to produce, in laboratory conditions, complex and innovative exopolysaccharides. In a previous study, the mesophilic strain Alteromonas macleodii subsp. fijiensis biovar deepsane was collected on the East Pacific Rise at 2600m depth. It was isolated from a polychaete annelid Alvinella pompejana and is able to synthesise and excrete the exopolysaccharide deepsane. Biological activities have been screened and some protective properties have been established. Deepsane is commercially available in cosmetics under the name of Abyssine (R) for soothing and reducing irritation of sensitive skin against chemical, mechanical and UVB aggression. This study presents structural data for this... |
| Tipo: Text |
Palavras-chave: Bacterial exopolysaccharide; Alteromonas macleodii; Structure determination; NMR spectroscopy; Mass spectrometry. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00095/20620/18536.pdf |
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| Camacho-chab, Juan Carlos; Guezennec, Jean; Chan-bacab, Manuel Jesus; Rios-leal, Elvira; Sinquin, Corinne; Muniz-salazar, Raquel; De La Rosa-garcia, Susana Del C.; Reyes-estebanez, Manuela; Ortega-morales, Benjamin Otto. |
| A previously reported bacterial bioemulsifier, here termed microbactan, was further analyzed to characterize its lipid component, molecular weight, ionic character and toxicity, along with its bioemulsifying potential for hydrophobic substrates at a range of temperatures, salinities and pH values. Analyses showed that microbactan is a high molecular weight (700 kDa), non-ionic molecule. Gas chromatography of the lipid fraction revealed the presence of palmitic, stearic, and oleic acids; thus microbactan may be considered a glycolipoprotein. Microbactan emulsified aromatic hydrocarbons and oils to various extents; the highest emulsification index was recorded against motor oil (96%). The stability of the microbactan-motor oil emulsion model reached its... |
| Tipo: Text |
Palavras-chave: Bioemulsifier; Emulsifying stability; Non-toxic; Microbacterium sp; Biotechnology. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00171/28260/26530.pdf |
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| Senni, Karim; Gueniche, Farida; Changotade, Sylvie; Septier, Dominique; Sinquin, Corinne; Ratiskol, Jacqueline; Lutomski, Didier; Godeau, Gaston; Guezennec, Jean; Colliec-jouault, Sylvia. |
| Biopolymers produced by marine organisms can offer useful tools for regenerative medicine. Particularly, HE800 exopolysaccharide (HE800 EPS) secreted by a deep-sea hydrothermal bacterium displays an interesting glycosaminoglycan-like feature resembling hyaluronan. Previous studies demonstrated its effectiveness to enhance in vivo bone regeneration and to support osteoblastic cell metabolism in culture. Thus, in order to assess the usefulness of this high-molecular weight polymer in tissue engineering and tissue repair, in vitro reconstructed connective tissues containing HE800 EPS were performed. We showed that this polysaccharide promotes both collagen structuring and extracellular matrix settle by dermal fibroblasts. Furthermore, from the native HE800... |
| Tipo: Text |
Palavras-chave: Marine hydrothermal bacteria; Glycosaminoglycan-mimetic; Collagen; Matrix metalloproteinases; Dermal fibroblast; Polysaccharides; Tissue engineering. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00138/24973/23072.pdf |
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| Goudenege, David; Boursicot, Vincent; Versigny, Typhaine; Bonnetot, Sandrine; Ratiskol, Jacqueline; Sinquin, Corinne; Lapointe, Gisele; Le Roux, Frederique; Delbarre-ladrat, Christine. |
| Vibrio diabolicus, a marine bacterium originating from deep-sea hydrothermal vents, produces the HE800 exopolysaccharide with high value for biotechnological purposes, especially for human health. Its genome was sequenced and analyzed; phylogenetic analysis using the core genome revealed V. diabolicus is close to another deep-sea Vibrio sp. (Ex25) within the Harveyi clade and Alginolyticus group. A genetic locus homologous to the syp cluster from Vibrio fischeri was demonstrated to be involved in the HE800 production. However, few genetic particularities suggest that the regulation of syp expression may be different in V. diabolicus. The presence of several types of glycosyltransferases within the locus indicates a capacity to generate diversity in the... |
| Tipo: Text |
Palavras-chave: Genome; Vibrio; Exopolysaccharide; Biosynthesis. |
| Ano: 2014 |
URL: https://archimer.ifremer.fr/doc/00241/35244/33892.pdf |
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| Delbarre-ladrat, Christine; Sinquin, Corinne; Lebellenger, Lou; Zykwinska, Agata; Colliec-jouault, Sylvia. |
| Although polysaccharides are ubiquitous and the most abundant renewable bio-components, their studies, covered by the glycochemistry and glycobiology fields, remain a challenge due to their high molecular diversity and complexity. Polysaccharides are industrially used in food products; human therapeutics fall into a more recent research field and pharmaceutical industry is looking for more and more molecules with enhanced activities. Glycosaminoglycans (GAGs) found in animal tissues play a critical role in cellular physiological and pathological processes as they bind many cellular components. Therefore, they present a great potential for the design and preparation of therapeutic drugs. On the other hand, microorganisms producing exopolysaccharides (EPS)... |
| Tipo: Text |
Palavras-chave: Marine-derivedpolysaccharide; Biodiversity; Biologicalactivity; Structure; Modification; GAG-mimetic; Biosynthesis. |
| Ano: 2014 |
URL: https://archimer.ifremer.fr/doc/00241/35250/33764.pdf |
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| Registros recuperados: 35 | |
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